Additional file 1 of A novel Chr1-miR-200 driven whole transcriptome signature shapes tumor immune microenvironment and predicts relapse in early-stage lung adenocarcinoma

Additional file 1: Figure S1. Repartition of EMT Score 76GS (A), miR-429 expression (B), mean miR-200 s chr12 expression (C), mean miR-200 s chr1 expression (D). MiR expression is evaluated by ΔΔCt value relative to 3 reference miRs (y axis)[32]. Figure S2 Kaplan–Meier curves of survival according to the EMT score estimated with the 76GS and divided in quartiles. In this cohort for DFS (A) and OS (B) and in TCGA data (C) for OS. Figure S3 Schematic workflow of supervised classification. Figure S4 Hierarchical clustering of 454 LUAD from the TCGA dataset based on the 493 genes reveals significant interactions between molecular subtypes (A) and a high correlation with WISP classification. The 4 clusters match the 3 clusters I, IIA/B identified in our cohort as demonstrated by a centroid correlation (B). Figure S5 Disease-Free Survival relative to WISP miR-200-signature defined as 50% of miR-200-signature-up = mir200-sign-up in our cohort adjusted by stage. Figure S6 Overall Survival relative to WISP miR-200-signature defined as 50% of miR-200-signature-up = mir200-sign-up in the TCGA LUAD cohort adjusted by stage. Figure S7 Survival relative to WISP miR-200-signature defined as 50% of miR-200-signature-up = mir200-sign-up among lung adenocarcinoma transcriptome available datasets. The p-values have been calculated by the log-rank test, HR = Hazard ratios estimated from a Cox univariate model, with the 95% confidence interval. Bild: https://doi.org/10.1038/nature04296 . Chang: https://doi.org/10.1002/cncr.25592 . Lee: https://doi.org/10.1158/1078-0432.CCR-07-4937 . Roepman: https://doi.org/10.1158/1078-0432.CCR-08-1258 . Shedden: https://doi.org/10.1038/nm.1790 . Takeuchi: https://doi.org/10.1200/JCO.2005.03.8224 . TomidaI: https://doi.org/10.1200/JCO.2008.19.7053 . Figure S8 Survival in early stages (I and II), compilation of the 7 lung adenocarcinoma available datasets.