Additional file 2 of Sex differences in the transcriptome of extracellular vesicles secreted by fetal neural stem cells and effects of chronic alcohol exposure

Additional file 2: Fig. S2. Principal Component Analysis of Cell and EV Samples Grouped by Pregnancy. Principal component analysis was performed on the 500 most variant RNA transcripts from 18 NSC parental cell samples (S2A) and their corresponding EV samples (S2B). This analysis shows that parental NSC samples, but not their secreted EVs, could be partly segregated within the 1st two principal components, by their pregnancy identity (i.e., which pregnant dam the fetal cells were derived from). This finding guided this study’s use of a repeated measures experimental design with pregnancy ID as a within-subjects factor for parametric statistical analyses.