Additional file 9 of Treatment with decitabine induces the expression of stemness markers, PD-L1 and NY-ESO-1 in colorectal cancer: potential for combined chemoimmunotherapy

Additional file 9: Supplement 8. Effect of DAC treatment on the expression of CD133, CD44 and Nanog in 1076 cells. Flow cytometry analysis showed that DAC treatment significantly increased the expression of CD44 (0.5% in untreated cells vs. 31.4% after DAC treatment) (A), CD133 (2.3% in untreated cells vs. 77.8% after DAC treatment) (B) and Nanog (0.5% in untreated cells vs. 44.3 % after DAC treatment) (C) in 1076 cells. The samples were acquired using BD LSR Fortessa and the data were analyzed using BD FACS DIVA software. (D) Western blot analysis showed that DAC treatment increased the expression of CD44, Nanog and KLF4 in both cell lines tested.