Additional file 1 of Anti-human immunodeficiency virus-1 activity of MoMo30 protein isolated from the traditional African medicinal plant Momordica balsamina

Additional file 1: Fig. S1. MoMo30-plant has chitinase activity. To determine if MoMo30-plant had chitinase activity, 5 µL of a 200 nM solution of MoMo30-plant was added to three different substrates (Sigma; cat # CS1030) in 100 µL of total volume a positive control is included for each substrate: 4-Methylumbelliferyl N,N′-diacetyl-β-D-triacetylchitotriose (Endo; endochitinase activity), 4-Methylumbelliferyl N,N′-diacetyl-β-D-chitobioside (Chito; exochitinase activity), and 4-Methylumbelliferyl N,N′-diacetyl-β-D-glucosaminide (Gluc; exochitinase activity). The assay is performed in an acidic environment (pH ~ 5.0) at 37 °C for 30 min. The assays were done in triplicate. The activity was measured as fluorescence and converted to activity in mg/mL. Fluorecent substrates require the least amount of time and were the most sensitive of the common methods used for chitinase activity [52].