Additional file 3 of Impaired neurogenesis and neural progenitor fate choice in a human stem cell model of SETBP1 disorder

Additional file 3: Fig. S2. Validation of cortical induction by qPCR. A qPCR analysis of pluripotency and telencephalic marker genes. RNA samples were harvested every 5 days from day 0 to 30. Levels of mRNA expression were normalized to day 0 mRNA levels. SETBP1-/- levels were normalized against WT levels. Data shown are Log2RQ levels. B qPCR analysis of neural stem cell and neuronal marker genes. RNA samples were harvested every 5 days from day 21 to 50. Levels of mRNA expression were normalized to day 21. SETBP1-/- levels were normalized against WT levels. Data shown are RQ levels. Data shown as mean ± s.e.m of two independent differentiations analyzed in triplicates. Student’s T test was used to compare the expression between the two lines (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001).