Panels A-B show MDR1 expression at the BBB

Copyright information:Taken from "Significance of MDR1 and multiple drug resistance in refractory human epileptic brain"BMC Medicine 2004;2():37-37.Published online 9 Oct 2004PMCID:PMC524356.Copyright © 2004 Marchi et al; licensee BioMed Central Ltd. Widespread GFAP immunoreactivity () co-localizes with MDR1 (). Nuclei are stained with DAPI. Note that both parenchymal and perivascular astrocytes express MDR1 (arrowheads), as do endothelial cells of the brain capillaries (). The box graph in B illustrates the percentage of GFAP-positive astrocytes () in samples from 11 patients (Table 1, 1–11) that also expressed MDR1. Data points (), mean value (), range () and standard errors (° SE) are shown together with the 50percentile value. Panels C-D show the neuronal expression of MDR1. Double immunostaining with MDR1 and two neuronal markers (neurofilament, and NeuN) reveals that MDR1 is expressed in a subpopulation of epileptic neurons. point to MDR1 negative neurons, while indicate the more frequently-occurring MDR1 positive neurons. Approximately 64% of cortical neurons (n = 264) were positive for MDR1. Quantitative analysis in D was obtained from 11 patients. Patient values (), mean (), range () and standard errors (± SE) are shown. The 50percentile is also indicated in the box graph.