Metabolomicsand arsenic-based liver injury methods reveal the mechanism of arsenic-induced liver injury in rats project

Arsenic (As) is an environmental poison and human carcinogen that poses a serious threat to human health, and long-term exposure to arsenic can cause liver damage. In order to explore the mechanism of As-induced liver injury in rats, a rat model of As poisoning was established, and metabolomics and ionomics methods were used to study the differences in liver metabolites and ion concentrations between As poisoned and control (Ctrl) rats. Through metabolomic analysis, 164 differentially expressed metabolites (DEMs) were identified in the As poisoning group and Ctrl group, among which nicotinate and nicotinamide metabolism, steroid hormone biosynthesis, taurine and subtaurine metabolism were significantly enriched. Arsenomics results showed that arsenic, cadmium (Cd), mercury (Hg) and manganese (Mn) were elevated in the arsenic poisoning group, and lead (Pb) and thallium (Tl) (p Correlation analysis of DEMs with differential ions showed that As, Cd and Hg were negatively correlated with androstenedione and estriol, while As was negatively correlated with progesterone (PROG), Cd and nicotinamide adenine dinucleotide (NAD). +), Mn and 5-L-glutamyl taurine were positively correlated. This study revealed the changes of liver metabolites and ion levels in rats with As toxicity model, and their relationship with the underlying mechanism of As-induced liver injury, which provided a reference for the study of the mechanism of As-induced hepatotoxicity.