Functional analysis of and conserved motifs

This analysis reveals the basis for divergence in tissue specificity. Panels a to e show a transgenic analysis of motifs by site specific mutations. Embryos injected with the corresponding constructs are shown at 24 hours post-fertilization (hpf) lateral view onto the trunk at the level of the midline. Shown are embryos injected with gfp-reporter constructs containing wild-type zebrafish , with mutated C1 region, mutated C2, mutated C3, mutated C4, and C4 replaced with medaka C4 (C4m). Panels g to k show a transgenic analysis of motifs. Shown are embryos injected with gfp-reporter constructs containing wild-type zebrafish , with mutated C1 and mutated C3, and with exchange of sequence with the zebrafish C2 and with the zebrafish C4. Stacked-column graphs show the quantification of the gfp expression, as described in Figure 3. Arrows and arrowheads point to floor plate and notochord cells, respectively. Lines on the left side indicate the level of the floor plate and notochord on the images. ect, ectopic; fp, floor plate; nt, notochord.Copyright information:Taken from "Functional diversification paralog enhancers identified by phylogenomic reconstruction"http://genomebiology.com/2007/8/6/R106Genome Biology 2007;8(6):R106-R106.Published online 8 Jun 2007PMCID:PMC2394741.