The Rv2540c DNA sequence encodes a bifunctional chorismate synthase-1

gradient from 0 to 1 M NaCl (dashed line). The absorbance was monitored at 445 nm (dotted line) and 280 nm (solid line). The first peak corresponds to free FMN, and the second peak corresponds to CS-FMNcomplex. Inset: UV-Vis spectrum ranging from 210 nm to 600 nm of the second pool (solid line) and Tris-HCl (dotted line). All characteristic peaks of FMNcan be observed (267 nm, 373 nm, and 445 nm).Copyright information:Taken from "The Rv2540c DNA sequence encodes a bifunctional chorismate synthase"http://www.biomedcentral.com/1471-2091/9/13BMC Biochemistry 2008;9():13-13.Published online 29 Apr 2008PMCID:PMC2386126.