Additional file 1 of Nelfinavir inhibition of Kaposi’s sarcoma-associated herpesvirus protein expression and capsid assembly
Supplementary Material 1. Fig. S1. Titration of KSHV virus produced from 5r219 cells. 5r219 cells (1 x106) were induced with doxycycline (DOX) or doxycycline plus 1 mM sodium butyrate (DOX+NB) and virus supernatants harvested, 72 h post-induction. The virus in the supernatants were concentrated by c...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Format: | Bild |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | Li, Maggie Smith, Barbara J. Lee, Jaeyeun Petr, Jennifer Anders, Nicole M. Wiseman, Robyn Rudek, Michelle A. Ambinder, Richard F. Desai, Prashant J. |
| description | Supplementary Material 1. Fig. S1. Titration of KSHV virus produced from 5r219 cells. 5r219 cells (1 x106) were induced with doxycycline (DOX) or doxycycline plus 1 mM sodium butyrate (DOX+NB) and virus supernatants harvested, 72 h post-induction. The virus in the supernatants were concentrated by centrifugation and resuspended in PBS overnight at +4ºC. The virus titer was quantitated by qPCR assays (A) and infection of Vero cell monolayers (B). Undiluted (Undil) as well as serial ten-fold dilutions were assayed using both methods. |
| doi_str_mv | 10.6084/m9.figshare.25341268 |
| format | Image |
| fullrecord | <record><control><sourceid>datacite_PQ8</sourceid><recordid>TN_cdi_datacite_primary_10_6084_m9_figshare_25341268</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_6084_m9_figshare_25341268</sourcerecordid><originalsourceid>FETCH-datacite_primary_10_6084_m9_figshare_253412683</originalsourceid><addsrcrecordid>eNqdzz1uAkEMhuFpKCLgBil8ATa7sEFQoigoEhJV-pHZ8WQtzZ_GEwQNyjW4Xk4SNgoXSOXi81O8Sj02dbWsV-2TX1eWP6THTNX8edE28-XqQV02xnDhGNCBZUfQQLSwJ2c54JEzcOj58PsxDDtMUfj76yogmLvocYYisWMsZKCnnEhu6lMg5ViIA9ApZRIZPAYDHSZhAzdE_uDOEzWy6ISmf3es2u3r-8vbzGDBjgvplNljPuum1kOG9mt9z9D3jMU_2Q-9mV90</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>image</recordtype></control><display><type>image</type><title>Additional file 1 of Nelfinavir inhibition of Kaposi’s sarcoma-associated herpesvirus protein expression and capsid assembly</title><source>DataCite</source><creator>Li, Maggie ; Smith, Barbara J. ; Lee, Jaeyeun ; Petr, Jennifer ; Anders, Nicole M. ; Wiseman, Robyn ; Rudek, Michelle A. ; Ambinder, Richard F. ; Desai, Prashant J.</creator><creatorcontrib>Li, Maggie ; Smith, Barbara J. ; Lee, Jaeyeun ; Petr, Jennifer ; Anders, Nicole M. ; Wiseman, Robyn ; Rudek, Michelle A. ; Ambinder, Richard F. ; Desai, Prashant J.</creatorcontrib><description>Supplementary Material 1. Fig. S1. Titration of KSHV virus produced from 5r219 cells. 5r219 cells (1 x106) were induced with doxycycline (DOX) or doxycycline plus 1 mM sodium butyrate (DOX+NB) and virus supernatants harvested, 72 h post-induction. The virus in the supernatants were concentrated by centrifugation and resuspended in PBS overnight at +4ºC. The virus titer was quantitated by qPCR assays (A) and infection of Vero cell monolayers (B). Undiluted (Undil) as well as serial ten-fold dilutions were assayed using both methods.</description><identifier>DOI: 10.6084/m9.figshare.25341268</identifier><language>eng</language><publisher>figshare</publisher><subject>Biochemistry ; Biotechnology ; Cancer ; Chemical Sciences not elsewhere classified ; FOS: Biological sciences ; FOS: Health sciences ; Infectious Diseases ; Medicine ; Mental Health ; Microbiology ; Molecular Biology ; Pharmacology ; Virology</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>780,1894</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6084/m9.figshare.25341268$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Li, Maggie</creatorcontrib><creatorcontrib>Smith, Barbara J.</creatorcontrib><creatorcontrib>Lee, Jaeyeun</creatorcontrib><creatorcontrib>Petr, Jennifer</creatorcontrib><creatorcontrib>Anders, Nicole M.</creatorcontrib><creatorcontrib>Wiseman, Robyn</creatorcontrib><creatorcontrib>Rudek, Michelle A.</creatorcontrib><creatorcontrib>Ambinder, Richard F.</creatorcontrib><creatorcontrib>Desai, Prashant J.</creatorcontrib><title>Additional file 1 of Nelfinavir inhibition of Kaposi’s sarcoma-associated herpesvirus protein expression and capsid assembly</title><description>Supplementary Material 1. Fig. S1. Titration of KSHV virus produced from 5r219 cells. 5r219 cells (1 x106) were induced with doxycycline (DOX) or doxycycline plus 1 mM sodium butyrate (DOX+NB) and virus supernatants harvested, 72 h post-induction. The virus in the supernatants were concentrated by centrifugation and resuspended in PBS overnight at +4ºC. The virus titer was quantitated by qPCR assays (A) and infection of Vero cell monolayers (B). Undiluted (Undil) as well as serial ten-fold dilutions were assayed using both methods.</description><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>Chemical Sciences not elsewhere classified</subject><subject>FOS: Biological sciences</subject><subject>FOS: Health sciences</subject><subject>Infectious Diseases</subject><subject>Medicine</subject><subject>Mental Health</subject><subject>Microbiology</subject><subject>Molecular Biology</subject><subject>Pharmacology</subject><subject>Virology</subject><fulltext>true</fulltext><rsrctype>image</rsrctype><creationdate>2024</creationdate><recordtype>image</recordtype><sourceid>PQ8</sourceid><recordid>eNqdzz1uAkEMhuFpKCLgBil8ATa7sEFQoigoEhJV-pHZ8WQtzZ_GEwQNyjW4Xk4SNgoXSOXi81O8Sj02dbWsV-2TX1eWP6THTNX8edE28-XqQV02xnDhGNCBZUfQQLSwJ2c54JEzcOj58PsxDDtMUfj76yogmLvocYYisWMsZKCnnEhu6lMg5ViIA9ApZRIZPAYDHSZhAzdE_uDOEzWy6ISmf3es2u3r-8vbzGDBjgvplNljPuum1kOG9mt9z9D3jMU_2Q-9mV90</recordid><startdate>20240305</startdate><enddate>20240305</enddate><creator>Li, Maggie</creator><creator>Smith, Barbara J.</creator><creator>Lee, Jaeyeun</creator><creator>Petr, Jennifer</creator><creator>Anders, Nicole M.</creator><creator>Wiseman, Robyn</creator><creator>Rudek, Michelle A.</creator><creator>Ambinder, Richard F.</creator><creator>Desai, Prashant J.</creator><general>figshare</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20240305</creationdate><title>Additional file 1 of Nelfinavir inhibition of Kaposi’s sarcoma-associated herpesvirus protein expression and capsid assembly</title><author>Li, Maggie ; Smith, Barbara J. ; Lee, Jaeyeun ; Petr, Jennifer ; Anders, Nicole M. ; Wiseman, Robyn ; Rudek, Michelle A. ; Ambinder, Richard F. ; Desai, Prashant J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_6084_m9_figshare_253412683</frbrgroupid><rsrctype>images</rsrctype><prefilter>images</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biochemistry</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>Chemical Sciences not elsewhere classified</topic><topic>FOS: Biological sciences</topic><topic>FOS: Health sciences</topic><topic>Infectious Diseases</topic><topic>Medicine</topic><topic>Mental Health</topic><topic>Microbiology</topic><topic>Molecular Biology</topic><topic>Pharmacology</topic><topic>Virology</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Maggie</creatorcontrib><creatorcontrib>Smith, Barbara J.</creatorcontrib><creatorcontrib>Lee, Jaeyeun</creatorcontrib><creatorcontrib>Petr, Jennifer</creatorcontrib><creatorcontrib>Anders, Nicole M.</creatorcontrib><creatorcontrib>Wiseman, Robyn</creatorcontrib><creatorcontrib>Rudek, Michelle A.</creatorcontrib><creatorcontrib>Ambinder, Richard F.</creatorcontrib><creatorcontrib>Desai, Prashant J.</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Li, Maggie</au><au>Smith, Barbara J.</au><au>Lee, Jaeyeun</au><au>Petr, Jennifer</au><au>Anders, Nicole M.</au><au>Wiseman, Robyn</au><au>Rudek, Michelle A.</au><au>Ambinder, Richard F.</au><au>Desai, Prashant J.</au><format>book</format><genre>unknown</genre><ristype>GEN</ristype><title>Additional file 1 of Nelfinavir inhibition of Kaposi’s sarcoma-associated herpesvirus protein expression and capsid assembly</title><date>2024-03-05</date><risdate>2024</risdate><abstract>Supplementary Material 1. Fig. S1. Titration of KSHV virus produced from 5r219 cells. 5r219 cells (1 x106) were induced with doxycycline (DOX) or doxycycline plus 1 mM sodium butyrate (DOX+NB) and virus supernatants harvested, 72 h post-induction. The virus in the supernatants were concentrated by centrifugation and resuspended in PBS overnight at +4ºC. The virus titer was quantitated by qPCR assays (A) and infection of Vero cell monolayers (B). Undiluted (Undil) as well as serial ten-fold dilutions were assayed using both methods.</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.25341268</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | DOI: 10.6084/m9.figshare.25341268 |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_datacite_primary_10_6084_m9_figshare_25341268 |
| source | DataCite |
| subjects | Biochemistry Biotechnology Cancer Chemical Sciences not elsewhere classified FOS: Biological sciences FOS: Health sciences Infectious Diseases Medicine Mental Health Microbiology Molecular Biology Pharmacology Virology |
| title | Additional file 1 of Nelfinavir inhibition of Kaposi’s sarcoma-associated herpesvirus protein expression and capsid assembly |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T23%3A09%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-datacite_PQ8&rft_val_fmt=info:ofi/fmt:kev:mtx:book&rft.genre=unknown&rft.au=Li,%20Maggie&rft.date=2024-03-05&rft_id=info:doi/10.6084/m9.figshare.25341268&rft_dat=%3Cdatacite_PQ8%3E10_6084_m9_figshare_25341268%3C/datacite_PQ8%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |