Additional file 1 of Nelfinavir inhibition of Kaposi’s sarcoma-associated herpesvirus protein expression and capsid assembly

Supplementary Material 1. Fig. S1. Titration of KSHV virus produced from 5r219 cells. 5r219 cells (1 x106) were induced with doxycycline (DOX) or doxycycline plus 1 mM sodium butyrate (DOX+NB) and virus supernatants harvested, 72 h post-induction. The virus in the supernatants were concentrated by centrifugation and resuspended in PBS overnight at +4ºC. The virus titer was quantitated by qPCR assays (A) and infection of Vero cell monolayers (B). Undiluted (Undil) as well as serial ten-fold dilutions were assayed using both methods.