Additional file 7 of E-cadherin variants associated with oral facial clefts trigger aberrant cell motility in a REG1A-dependent manner

Additional file 7: Supplementary Figure 1. E-cadherin structure, expression and localization in HDGC and OFC cell mutants. (A) 3D visualization of E-cadherin structure with Mol* Viewer, highlighting the D254 and P373 positions. (B) Impact of D254H, P373L, D254N and P373R variants in protein conformation. (C) Protein levels were analysed by Western Blot in (C-D) CHO and (E-F) AGS cells transfected with vectors encoding wild-type E-cadherin and the different variants, or the empty vector (Mock) as a control. α-Tubulin was used as a loading reference. Band intensity was quantified and normalized against wild-type cells. (G) Immunofluorescence was applied to evaluate protein localization. E-cadherin is shown in green and nuclei were counterstained with DAPI (blue). Graphs show quantification of signal intensities along contiguous cells (internuclear profiles). Position 1 and 100 correspond to the geometric centers of nucleus 1 and nucleus 2, respectively. Position 50 represents the plasma membrane.