Additional file 2 of GABA induced by sleep deprivation promotes the proliferation and migration of colon tumors through miR-223-3p endogenous pathway and exosome pathway

Additional file 2: Figure. S2. GABA inhibits ubiquitination of cMYC and promotes proliferation and migration of CRC. (A) A cluster heatmap of expression of GABARAP, GABBR1 and GABBR2 in 30 paired samples from GSE database. (B) The expression of GABARAP and GABBR2 in paired colon cancer tissue and paracancer tissue was detected by qRT-PCR. (C) The expression of GABBR2 in HCT116, SW480, LoVo and DLD1 cells was detected by Western blot. (D) The expreesion of E-cadherin, N-cadherin, Vimentin, CyclinE and Cyclin D1 in SW480 and LoVo cells treated by different concentration of GABA was detected by Western blot. (E) Western blot revealed that GABA promoted the proliferation and migration in SW480 and LoVo cells. (F) The wound healing assays showed that cMYC knockdown reversed the effects of GABA-induced promotion of migration in colon cancer cells.