Additional file 1 of Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models
Additional file 1: Supplemental Figure S1. Stratum corneum formation and lifespan of HEEs. (A) H&E and DAPI staining of two HEE donors that were topically applied with LY for 2.5 h on different days of the air-liquid interface (ALI) to evaluate stratum corneum penetration (images represent eight...
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| creator | Rikken, Gijs Meesters, Luca D. Jansen, Patrick A. M. Rodijk-Olthuis, Diana van Vlijmen-Willems, Ivonne M. J. J. Niehues, Hanna Smits, Jos P. H. Oláh, Peter Homey, Bernhard Schalkwijk, Joost Zeeuwen, Patrick L. J. M. van den Bogaard, Ellen H. |
| description | Additional file 1: Supplemental Figure S1. Stratum corneum formation and lifespan of HEEs. (A) H&E and DAPI staining of two HEE donors that were topically applied with LY for 2.5 h on different days of the air-liquid interface (ALI) to evaluate stratum corneum penetration (images represent eight biological keratinocyte donors). (B) Protein expression of the proliferation marker Ki-67, differentiation markers filaggrin (FLG) and keratin 10 (K10) and the AMP SKALP/elafin of a HEE at day 25 of the ALI. (C) H&E staining of HEEs harvested at day 25 and 30 of the ALI to investigate the lifespan of the culture. Scale bar = 100 µm. |
| doi_str_mv | 10.6084/m9.figshare.24329773 |
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(B) Protein expression of the proliferation marker Ki-67, differentiation markers filaggrin (FLG) and keratin 10 (K10) and the AMP SKALP/elafin of a HEE at day 25 of the ALI. (C) H&E staining of HEEs harvested at day 25 and 30 of the ALI to investigate the lifespan of the culture. 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(B) Protein expression of the proliferation marker Ki-67, differentiation markers filaggrin (FLG) and keratin 10 (K10) and the AMP SKALP/elafin of a HEE at day 25 of the ALI. (C) H&E staining of HEEs harvested at day 25 and 30 of the ALI to investigate the lifespan of the culture. Scale bar = 100 µm.</description><subject>FOS: Biological sciences</subject><subject>Microbiology</subject><fulltext>true</fulltext><rsrctype>image</rsrctype><creationdate>2023</creationdate><recordtype>image</recordtype><sourceid>PQ8</sourceid><recordid>eNqdjzFOxDAQRd1QIOAGFHOBhGQTsaREwIqKantrsMfJCDsT2QPSHoFbb1bsXoDqS___Vzxj7tumfmye-oc01IHHMmGmetN3m2G77a7N77P3rCwzRggcCVqQAB_yQxES6SReooxMBYJkmKRoldhl-STgWSmjO8EFcPbwN7AkqhTzSEoedFo_C30ru7IS0L2C5BFn0cPCDsrX2iXxFMutuQoYC92d88b0u7f9y3vlUdGxkl0yJ8wH2zb2ZGTTYC9G9mLU_RM7AmOeYls</recordid><startdate>20231018</startdate><enddate>20231018</enddate><creator>Rikken, Gijs</creator><creator>Meesters, Luca D.</creator><creator>Jansen, Patrick A. 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M.</creatorcontrib><creatorcontrib>van den Bogaard, Ellen H.</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Rikken, Gijs</au><au>Meesters, Luca D.</au><au>Jansen, Patrick A. M.</au><au>Rodijk-Olthuis, Diana</au><au>van Vlijmen-Willems, Ivonne M. J. J.</au><au>Niehues, Hanna</au><au>Smits, Jos P. H.</au><au>Oláh, Peter</au><au>Homey, Bernhard</au><au>Schalkwijk, Joost</au><au>Zeeuwen, Patrick L. J. M.</au><au>van den Bogaard, Ellen H.</au><format>book</format><genre>unknown</genre><ristype>GEN</ristype><title>Additional file 1 of Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models</title><date>2023-10-18</date><risdate>2023</risdate><abstract>Additional file 1: Supplemental Figure S1. Stratum corneum formation and lifespan of HEEs. 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| title | Additional file 1 of Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models |
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