Additional file 1 of Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models

Additional file 1: Supplemental Figure S1. Stratum corneum formation and lifespan of HEEs. (A) H&E and DAPI staining of two HEE donors that were topically applied with LY for 2.5 h on different days of the air-liquid interface (ALI) to evaluate stratum corneum penetration (images represent eight biological keratinocyte donors). (B) Protein expression of the proliferation marker Ki-67, differentiation markers filaggrin (FLG) and keratin 10 (K10) and the AMP SKALP/elafin of a HEE at day 25 of the ALI. (C) H&E staining of HEEs harvested at day 25 and 30 of the ALI to investigate the lifespan of the culture. Scale bar = 100 µm.