Supplementary information for the manuscript titled "Orchid fruit and root movement analysed with 2D photographs and a bioinformatics pipeline for processing sequential 3D scans"

The Supporting Information comprises 11 appendices, which include:Appendix S1. 3D CT scan contrast test of different media in which Erycina pusilla was grown: (A) gelrite; B) glass beads; C) sand; D) solid agar; E) Sphagnum moss; and F) cotton). Scale bar: 1 cm.Appendix S2. CT scan protocol of young, mature, early, and late flowering plants of Erycina pusilla.Appendix S3. Scanning and reconstructing images of Erycina pusilla plants with SkyScan1172 Micro CT: a step-by-step guide.Appendix S4. Response of five plants exposed to weekly X-ray radiation compared to four control plants not exposed to X-rays.Appendix S5. Effect of repeated exposure to X-rays during CT-scanning over 19 weeks on development of young, mature, early, and fully flowering plants of Erycina pusilla compared with control plants not exposed to any X-rays.Appendix S6. Development of Erycina pusilla from seed to flowering stage recorded in the tissue cultivation laboratory of Naturalis Biodiversity Center. A) Leaf-like protocorm visible 28 days after germination (DAG); B) First development of roots at 35 DAG; C-G) seedling development 42-70 DAG; H-I) Vegetative plant stages 77-84.DAG; J-K) inflorescence development and initiation of resupination ± 180 degrees of the flower 91-98 DAG; L-M) Elongation of inflorescence stalk 105-112 DAG; O) Floral bud emerging from bract 119 DAG; P-Q) 30 degrees bending of pedicel until (R-S) flower opens 126-133 DAG. Scale bar: 1 cm.Appendix S7. Resupination of roots of Erycina pusilla after different exposure times to x-rays. Abbreviations: Root S-1: root of seedling; Root NF1-1: root of the first replication non-flowering plant; Root NF2-1= root number one of second replication of non-flowering plant; Root NF2-2= root number two of second replication of non-flowering plant; Root NF2-3= root number three of second replication of non-flowering plants.Appendix S8. Cell number data of P. equestris fruits analysed.Appendix S9. Area measurement data of P. equestris fruits analysed.Appendix S10. Number of cells of each valve in eight developmental stages of Phalaenopsis equestris fruits grown in the tissue cultivation laboratory of Naturalis Biodiversity Center. The number of cells is shown on the vertical axis, and the fruit developmental stages (days after pollination-DAP) are displayed on the horizontal axis. Abbreviations: Lab= fertile valve derived from labellum; P1= fertile valve derived from the first petal; P2= fertile valve derived from the second petal;