An embryological twist in zebra fish embryo, as revealed by cellular movement patterns

SourceThese videos belong to the appendix of de Lussanet & Osse (2012). The publisher (Brill, Leiden, NL) no longer provides a public link. The copyright rests with the authors.Appendix A.1. Additional movie 1: low.movSupplementary movie A.1 is based on the supplementary movie S2 of Keller et al. (2008), (with permission) © Keller www.embl.de/digitalembryo. For this we extracted a number of movie frames from their supplementary Movie S2. During this period (865-1000 min) the cells that will form the prospective eye cell masses, migrate clockwise (encircled blue and red respectively), whereas the future mid- and hindbrain rotate anti-clockwise (green arrows). The prospective right eye cell mass (blue) is initially invis- ible because it is hidden by the embryo. Starting from 865 min, every 15-min a frame was marked. For accurate play- back, please select “Play All Frames” in the QuickTime Player. Note that the beginning and end of the movie are sped-up.Appendix A.2. Additional movie 2: selected.movMovie A.2 is a sped-up version of Additional movie 1, to show more clearly the axial compensatory movements. Advice for repeated playing: select “Loop” in your Quick- time player.Referencesde Lussanet, Marc H. E. and Osse, Jan W. M. (2012) An ancestral axial twist explains the contralateral forebrain and the optic chiasm in vertebrates. Animal Biol., 62(2):193–216. doi: 10.1163/157075611X617102. URL http://arxiv.org/abs/1003.1872.Philipp J. Keller, Annette D. Schmidt, Joachim Wittbrodt, and Ernst H. K. Stelzer. Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy. Science, 322(5904):1065–1069, 2008. doi: 10.1126/sci- ence.1162493.