Differences in fusion protein localization during pseudopod formation

GFP-FLN (A-E) and GFP-FLNABD (F-J) showed similar patterns of localization during pseudopod formation. Both probes maintain a cortical localization as the new protrusion begins to develop between the plasma membrane and the actin cortex (A and F) as the developing protrusion matures the cortex eventually breaks down and a new cortex forms at the periphery of the new protrusion (E and J). GFP-αA (K-N) and GFP-αAABD (O-R) showed no localization to the actin cortex during or after maturation of the pseudopod. Both probes remain cytoplasmic until the protrusion starts (K and O) and remains localized to the actin within the protrusion as the protrusion grows (L-N and P-R).Copyright information:Taken from "Actin binding domains direct actin-binding proteins to different cytoskeletal locations"http://www.biomedcentral.com/1471-2121/9/10BMC Cell Biology 2008;9():10-10.Published online 13 Feb 2008PMCID:PMC2275727.