Additional file 4 of Fructose promotes angiogenesis by improving vascular endothelial cell function and upregulating VEGF expression in cancer cells

Additional file 4: Fig. S3. Effects of fructose on VEC viability under hypoxic condition. a, CCK-8 analysis of the viability of SVEC4-10 cells cultured in glucose-free medium containing different concentrations of fructose, supplemented with 10% FBS or DFBS. b, Cell death analysis of SVEC4-10 cells cultured for 24 h in glucose-free medium with or without fructose (20mM). c, CCK-8 analysis of the viability of SVEC4-10 and HUVEC cells cultured in 20 mM fructose-containing medium in the presence of 2, 5-AM (3 mM), 2-DG (2 mM) or KHK inhibitor (1 μM) for 24 h under hypoxic condition. d, CCK-8 analysis of the viability of SVEC4-10 and HUVEC cells cultured in 20 mM glucose-containing medium in the presence of 2, 5-AM (3 mM), 2-DG (2 mM) or KHK inhibitor (1 μM) for 24 h under hypoxic condition. All data are expressed as the mean ± SD; ns, non-significant; *p < 0.05; **p < 0.01. n = 3.