Scaling behaviour and control of nuclear wrinkles

This repository contains some of the data presented in the article 'Scaling behaviour and control of nuclear wrinkles' by the same authors in Nature Physics (2023). For more details on the methods, see the accompanying manuscript.plotData_spreadsheets.zip contains the experimental data used in plots.figure_tifs.zip contains full-resolution tif versions of the images shown in the article and supplementary material.point_clouds.zip contains the point cloud coordinates extracted from the 3d microscopy images. The point clouds represent the location of GFP-tagged nuclear pore complexes, and as such are a reliable proxy to infer the spatial organization of the nuclear envelope of the imaged cells. The included MATLAB code processes those point clouds and represents them using scalar spherical harmonics, outputting MATLAB cell arrays containing the spherical harmonic coefficients along with other experimentally relevant information. To execute, open and run `wrinklesMain.m' in MATLAB. You can select which folder to process, where to place the output and more by modifying the code.The data is split into 4 folders:1. WT_allData_through2022-03-01 contains the wild-type data from a range of developmental stages.0pt5xOsmolarity contains the hypotonic shock data1pt5xOsmolarity contains the hypertonic shock dataallColchicine contains the data from the microtubule inhibition assays.For simulation code, we point to https://github.com/NicoRomeo/d3shell.