Additional file 2 of Umbelliferone and eriodictyol suppress the cellular entry of SARS-CoV-2

Additional file 2: Fig. S2 A. argyi, eriodictyol, and umbelliferone did not affect the cellular entry of VSVG-pseudotyped lentivirus.HEK-293T cells expressing ACE2 were infected with VSVG-pseudotyped lentivirus encoding luciferase after treatments with A. argyi, eriodictyol, or umbelliferone at the indicated concentrations for 2 days, and the luciferase activities were subsequently measured to determine the infection rate with the pseudoviruses. Additionally, the cell viability also was examined in CCK-8 assays. Camostat, naphthofluorescein, and cepharanthinewere used as the positive controls for the inhibitions of TMPRSS2 and furin activities and the binding between S protein and ACE2, respectively. Data were shown as mean±SEM from three independent experiments with triplicates. *** p