Additional file 4 of RP11-296E3.2 acts as an important molecular chaperone for YBX1 and promotes colorectal cancer proliferation and metastasis by activating STAT3

Additional file 4: Figure S4. RNA pulldown and RIP assays of RP11-296E3.2 and the effect of YBX1 on CRC stage. (A) An RNA pulldown assay was performed using RP11-296E3.2 sense and antisense RNAs in HCT116 cells, followed by silver staining. The red arrow indicates YBX1. (B) Representative ELAVL1 and YBX1 peptides identified by MS. (C) WB analysis was performed to verify the results of the ELAVL1 and RACK1 RNA pulldown assay. The RP11-296E3.2 antisense RNA was used as a control. (D) RIP experiments were performed with ELAVL1.