Additional file 9 of Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway

Additional file 9: Figure S9. Restoration of O-GlcNAcylation inhibits astrocyte activation and inflammation, and improves cognitive function of Ogt deficient mice.Schematic illustration of GlcNAc administration strategy. 2.5-month-old Ctrl and AD mice were administrated with salineand GlcNAcfor 6 weeks, and mice were sacrificed for assays.Representative images of O-GlcNAcylation and Iba1 immunostaining with brain sections of Ctrl and cKO mice treated with salineand GlcNAc, respectively. Scale bar, 100 μm.Quantification results show that the level of of O-GlcNAcylation fluorescence intensitysignificantly decreased in the hippocampus region of AD mice compared to Ctrl mice, which was significantly increased by GlcNAc administration. The intensity of Iba1 fluorescence was also significantly reduced after GlcNAc administration. n = 5 mice per group. Values represent mean ± SEM; *p