Additional file 7 of Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway
Additional file 7: Figure S7. Ogt interacts with NF-κB and catalyzes the O-GlcNAcylation of NF-κB.Schematic illustration of O-GlcNAcylation sites of p65 on S374, S370, S384 and S396 in N2a cells identified by MS/MS analysis. nanoLC-LTQ-CID was used to mapping the sites of O-GlcNAcylation on p65. The...
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| creator | Dong, Xiaoxue Shu, Liqi Zhang, Jinyu Yang, Xu Cheng, Xuejun Zhao, Xingsen Qu, Wenzheng Zhu, Qiang Shou, Yikai Peng, Guoping Sun, Binggui Yi, Wen Shu, Qiang Li, Xuekun |
| description | Additional file 7: Figure S7. Ogt interacts with NF-κB and catalyzes the O-GlcNAcylation of NF-κB.Schematic illustration of O-GlcNAcylation sites of p65 on S374, S370, S384 and S396 in N2a cells identified by MS/MS analysis. nanoLC-LTQ-CID was used to mapping the sites of O-GlcNAcylation on p65. The matched fragment ions are labeled in y and b.IP-WB assayand quantification results show that mutation of single potential O-GlcNAcylation sites led to a significant decrease in the O-GlcNAcylation level of p65in N2a cells, and only the mutation of S384 induced a significant increase of p-p65. n = 3 independent experiments. Values represent mean ± SEM; *p |
| doi_str_mv | 10.6084/m9.figshare.23564885 |
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Ogt interacts with NF-κB and catalyzes the O-GlcNAcylation of NF-κB.Schematic illustration of O-GlcNAcylation sites of p65 on S374, S370, S384 and S396 in N2a cells identified by MS/MS analysis. nanoLC-LTQ-CID was used to mapping the sites of O-GlcNAcylation on p65. The matched fragment ions are labeled in y and b.IP-WB assayand quantification results show that mutation of single potential O-GlcNAcylation sites led to a significant decrease in the O-GlcNAcylation level of p65in N2a cells, and only the mutation of S384 induced a significant increase of p-p65. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F = 51.62 forand F = 74.06 for.IP followed by WB assay results showed the level of GSK3β showed no difference in the hippocampus of Ctrl and cKO mice, and the interaction between p65 and GSK3β was significantly increased in the hippocampus of Ctrl and cKO mice. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.co-IP followed by WB assay showed no direct interaction between Gsk3β and Ogt.]]></description><identifier>DOI: 10.6084/m9.figshare.23564885</identifier><language>eng</language><publisher>figshare</publisher><subject>Biochemistry ; Cancer ; Cell Biology ; Developmental Biology ; FOS: Biological sciences ; FOS: Clinical medicine ; FOS: Health sciences ; Immunology ; Infectious Diseases ; Mental Health ; Neuroscience ; Physiology ; Science Policy ; Virology</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>780,1894</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.6084/m9.figshare.23564885$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Dong, Xiaoxue</creatorcontrib><creatorcontrib>Shu, Liqi</creatorcontrib><creatorcontrib>Zhang, Jinyu</creatorcontrib><creatorcontrib>Yang, Xu</creatorcontrib><creatorcontrib>Cheng, Xuejun</creatorcontrib><creatorcontrib>Zhao, Xingsen</creatorcontrib><creatorcontrib>Qu, Wenzheng</creatorcontrib><creatorcontrib>Zhu, Qiang</creatorcontrib><creatorcontrib>Shou, Yikai</creatorcontrib><creatorcontrib>Peng, Guoping</creatorcontrib><creatorcontrib>Sun, Binggui</creatorcontrib><creatorcontrib>Yi, Wen</creatorcontrib><creatorcontrib>Shu, Qiang</creatorcontrib><creatorcontrib>Li, Xuekun</creatorcontrib><title>Additional file 7 of Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway</title><description><![CDATA[Additional file 7: Figure S7. Ogt interacts with NF-κB and catalyzes the O-GlcNAcylation of NF-κB.Schematic illustration of O-GlcNAcylation sites of p65 on S374, S370, S384 and S396 in N2a cells identified by MS/MS analysis. nanoLC-LTQ-CID was used to mapping the sites of O-GlcNAcylation on p65. The matched fragment ions are labeled in y and b.IP-WB assayand quantification results show that mutation of single potential O-GlcNAcylation sites led to a significant decrease in the O-GlcNAcylation level of p65in N2a cells, and only the mutation of S384 induced a significant increase of p-p65. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F = 51.62 forand F = 74.06 for.IP followed by WB assay results showed the level of GSK3β showed no difference in the hippocampus of Ctrl and cKO mice, and the interaction between p65 and GSK3β was significantly increased in the hippocampus of Ctrl and cKO mice. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.co-IP followed by WB assay showed no direct interaction between Gsk3β and Ogt.]]></description><subject>Biochemistry</subject><subject>Cancer</subject><subject>Cell Biology</subject><subject>Developmental Biology</subject><subject>FOS: Biological sciences</subject><subject>FOS: Clinical medicine</subject><subject>FOS: Health sciences</subject><subject>Immunology</subject><subject>Infectious Diseases</subject><subject>Mental Health</subject><subject>Neuroscience</subject><subject>Physiology</subject><subject>Science Policy</subject><subject>Virology</subject><fulltext>true</fulltext><rsrctype>image</rsrctype><creationdate>2023</creationdate><recordtype>image</recordtype><sourceid>PQ8</sourceid><recordid>eNqdj0FuwjAQRb3pomq5QRdzgaShBBqWtAK6gg17a7AdeyQ7RvZQFKkn6yF6phIBF2A1o_n_afSEeBlX5axq6tcwL1uy2WEy5dtkOqubZvoofhZaE1Ps0ENL3sA7xBa2lotgNCEbDdti7dVmoXqPQxGoc7QnzoCZU1Q9m_OqmL4vMbsUj9ZBiPo4EJ2Fzar4-_2ATPb8ZjgckN0J-2fx0KLPZnSdT6JeLXefX4VGRkVs5CFRwNTLcSUHCRnm8iYhbxKTO7F_COpdtQ</recordid><startdate>20230623</startdate><enddate>20230623</enddate><creator>Dong, Xiaoxue</creator><creator>Shu, Liqi</creator><creator>Zhang, Jinyu</creator><creator>Yang, Xu</creator><creator>Cheng, Xuejun</creator><creator>Zhao, Xingsen</creator><creator>Qu, Wenzheng</creator><creator>Zhu, Qiang</creator><creator>Shou, Yikai</creator><creator>Peng, Guoping</creator><creator>Sun, Binggui</creator><creator>Yi, Wen</creator><creator>Shu, Qiang</creator><creator>Li, Xuekun</creator><general>figshare</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20230623</creationdate><title>Additional file 7 of Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway</title><author>Dong, Xiaoxue ; Shu, Liqi ; Zhang, Jinyu ; Yang, Xu ; Cheng, Xuejun ; Zhao, Xingsen ; Qu, Wenzheng ; Zhu, Qiang ; Shou, Yikai ; Peng, Guoping ; Sun, Binggui ; Yi, Wen ; Shu, Qiang ; Li, Xuekun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_6084_m9_figshare_235648853</frbrgroupid><rsrctype>images</rsrctype><prefilter>images</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biochemistry</topic><topic>Cancer</topic><topic>Cell Biology</topic><topic>Developmental Biology</topic><topic>FOS: Biological sciences</topic><topic>FOS: Clinical medicine</topic><topic>FOS: Health sciences</topic><topic>Immunology</topic><topic>Infectious Diseases</topic><topic>Mental Health</topic><topic>Neuroscience</topic><topic>Physiology</topic><topic>Science Policy</topic><topic>Virology</topic><toplevel>online_resources</toplevel><creatorcontrib>Dong, Xiaoxue</creatorcontrib><creatorcontrib>Shu, Liqi</creatorcontrib><creatorcontrib>Zhang, Jinyu</creatorcontrib><creatorcontrib>Yang, Xu</creatorcontrib><creatorcontrib>Cheng, Xuejun</creatorcontrib><creatorcontrib>Zhao, Xingsen</creatorcontrib><creatorcontrib>Qu, Wenzheng</creatorcontrib><creatorcontrib>Zhu, Qiang</creatorcontrib><creatorcontrib>Shou, Yikai</creatorcontrib><creatorcontrib>Peng, Guoping</creatorcontrib><creatorcontrib>Sun, Binggui</creatorcontrib><creatorcontrib>Yi, Wen</creatorcontrib><creatorcontrib>Shu, Qiang</creatorcontrib><creatorcontrib>Li, Xuekun</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Dong, Xiaoxue</au><au>Shu, Liqi</au><au>Zhang, Jinyu</au><au>Yang, Xu</au><au>Cheng, Xuejun</au><au>Zhao, Xingsen</au><au>Qu, Wenzheng</au><au>Zhu, Qiang</au><au>Shou, Yikai</au><au>Peng, Guoping</au><au>Sun, Binggui</au><au>Yi, Wen</au><au>Shu, Qiang</au><au>Li, Xuekun</au><format>book</format><genre>unknown</genre><ristype>GEN</ristype><title>Additional file 7 of Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway</title><date>2023-06-23</date><risdate>2023</risdate><abstract><![CDATA[Additional file 7: Figure S7. Ogt interacts with NF-κB and catalyzes the O-GlcNAcylation of NF-κB.Schematic illustration of O-GlcNAcylation sites of p65 on S374, S370, S384 and S396 in N2a cells identified by MS/MS analysis. nanoLC-LTQ-CID was used to mapping the sites of O-GlcNAcylation on p65. The matched fragment ions are labeled in y and b.IP-WB assayand quantification results show that mutation of single potential O-GlcNAcylation sites led to a significant decrease in the O-GlcNAcylation level of p65in N2a cells, and only the mutation of S384 induced a significant increase of p-p65. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F = 51.62 forand F = 74.06 for.IP followed by WB assay results showed the level of GSK3β showed no difference in the hippocampus of Ctrl and cKO mice, and the interaction between p65 and GSK3β was significantly increased in the hippocampus of Ctrl and cKO mice. n = 3 independent experiments. Values represent mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; unpaired Student’s t-test.co-IP followed by WB assay showed no direct interaction between Gsk3β and Ogt.]]></abstract><pub>figshare</pub><doi>10.6084/m9.figshare.23564885</doi><oa>free_for_read</oa></addata></record> |
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| title | Additional file 7 of Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway |
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