Additional file 7 of Ogt-mediated O-GlcNAcylation inhibits astrocytes activation through modulating NF-κB signaling pathway

Additional file 7: Figure S7. Ogt interacts with NF-κB and catalyzes the O-GlcNAcylation of NF-κB.Schematic illustration of O-GlcNAcylation sites of p65 on S374, S370, S384 and S396 in N2a cells identified by MS/MS analysis. nanoLC-LTQ-CID was used to mapping the sites of O-GlcNAcylation on p65. The matched fragment ions are labeled in y and b.IP-WB assayand quantification results show that mutation of single potential O-GlcNAcylation sites led to a significant decrease in the O-GlcNAcylation level of p65in N2a cells, and only the mutation of S384 induced a significant increase of p-p65. n = 3 independent experiments. Values represent mean ± SEM; *p