Cone Eestimated from the reversal of I

Copyright information:Taken from "Chloride equilibrium potential in salamander cones"BMC Neuroscience 2004;5():53-53.Published online 5 Dec 2004PMCID:PMC539262.Copyright © 2004 Thoreson and Bryson; licensee BioMed Central Ltd. A) Itail current was activated by applying a 500 ms step from -78 to -18 mV during a gramicidin perforated patch whole cell recording from a rod. A ramp voltage protocol (-98 to +52 mV, 1 mV/ms) was applied during the tail current and begun 25 ms after termination of the step. B. The same protocol was then repeated in the presence of niflumic acid (0.1 mM) to inhibit I. C. The ramp current/voltage relationship obtained in control medium (A) was subtracted from that obtained in the presence of niflumic acid (B) to yield a niflumic acid-sensitive difference current that reversed in this cell around -46 mV (after correcting for a liquid junction potential of -8 mV).