Additional file 1 of OLMALINC/OCT4/BMP2 axis enhances osteogenic-like phenotype of renal interstitial fibroblasts to participate in Randall’s plaque formation

Additional file 1: Fig. S1. (A). The immunohistochemistry (IHC) staining intensity of OCT4 and osteogenic markers (RUNX2, OCN) was quantified by average grey value using Image J. The average grey value ranged from 0 to 254. A black, dark-stained area was set as a grey value of 0, and a white, unstained area was set as a grey value of 254, causing an inverse correlation of the average gray value with staining intensity (Randall’s Plaques (RP), n = 8; normal renal papillae (NRP), n = 8). (B) Representative immunofluorescence co-staining images of Vimentin (Green) and RUNX2 (Red) in RP (n = 6) and NRP (n = 6). (C) Representative immunofluorescence co-staining images of Vimentin (Green) and OCN (Red) in RP (n = 6) and NRP (n = 6). (D) The Vimentin (VIM) co-localized with OCT4 or osteogenic markers (RUNX2, OCN) was quantified by calculating the ratio of co-localized VIM to total VIM using the confocal microscope system (RP = 6; NRP = 6).