Assessment of a Biochip Immunoassay for Multi-Mycotoxins Screening in Rice

The purpose of this study is the validation of an immunoassay for semi-quantitative screening of various mycotoxins: Aflatoxin B1 (AFB1), Aflatoxin G1 (AFG1), Ochratoxin A (OTA), Zearalenone (ZEA), Toxin T-2 and HT-2, Deoxynivalenol (DON) and Fumonisins (FB1+FB2) in rice samples. According to validation results, spiked rice samples showed low false results (5% of false negatives and 5% of false positives for fumonisins (FB1 and FB2), 5% of false negatives for ZEA, OTA, AFB1, T2HT2 and DON), in agreement with European Union legislation performance criteria [1].