Experimental design and DNA damage

Copyright information:Taken from "Responses of hyperthermophilic crenarchaea to UV irradiation"http://genomebiology.com/2007/8/10/R220Genome Biology 2007;8(10):R220-R220.Published online 11 Oct 2007PMCID:PMC2246294. Illustration of the experiment design. After growth to early log phase, cells were placed in Petri dishes and exposed to 200 J/mUV irradiation, then allowed to recover in the dark at 80°C. Arrows indicate sampling time points. Optical density measurement of UV-treated (red) and control (blue) cultures. Cells were exposed to UV at time zero. Generation and removal of CPDs in chromosomal DNA. DNA damage was measured by ELISA using a primary antibody specific for CPDs. Data were normalized so that the signal at time zero (immediately after UV irradiation) represented 100% unrepaired CPDs. Each data point represents the mean from six experimental replicates and error bars show the standard deviation of each data set.