Intraspecific diversity in the mechanisms underlying abamectin resistance in a cosmopolitan pest

Villacis-Perez et al. (2023) uncovers the complex basis of abamectin resistance, and highlights the intraspecific diversity of genetic mechanisms underlying resistance in Tetranychus urticae. This study used experimental evolution in combination with a population-level based bulked segregant analysis (BSA) and with next-generation sequencing to map quantitative trait loci (QTL) associated with abamectin resistance in two genetically unrelated strains of T. urticae, ROS-ITi and MAR-ABi (Kurlovs et al., 2019). Results reveal how unrelated populations of a single arthropod species respond to abamectin selection, the (polygenic) structure of abamectin resistance, and the genes and mechanisms likely to underlie abamectin resistance. The Supplementary Data for Villacis-Perez et al. (2023) comprises: Data S1: genomic VCF (gzipped) used as input for the RUN_BSA1.02.py script available at https://github.com/rmclarklab/BSA (Kurlovs et al. 2019, PMID: 31499416); Data S2: RUN_BSA1.02.py commands using the gvcf in Data S1 as input.; Data S3: output of the RUN_BSA1.02.py script for aBSA (MAR-ABi); Data S4: output of the RUN_BSA1.02.py script for gBSA ROS-ITi.