Bacterial flora-typing with targeted, chip-based Pyrosequencing-7

Bacterial flora-typing with targeted, chip-based Pyrosequencing-7

Copyright information:Taken from "Bacterial flora-typing with targeted, chip-based Pyrosequencing"http://www.biomedcentral.com/1471-2180/7/108BMC Microbiology 2007;7():108-108.Published online 30 Nov 2007PMCID:PMC2244631. by amplification of 16S rDNA using universal primers. These fragment...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Sundquist, Andreas, Saharnaz Bigdeli, Jalili, Roxana, Druzin, Maurice L, Waller, Sarah, Pullen, Kristin M, El-Sayed, Yasser Y, M Mark Taslimi, Batzoglou, Serafim, Ronaghi, Mostafa
Format: Bild
Sprache:eng
Schlagworte:
Uncategorized
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Copyright information:Taken from "Bacterial flora-typing with targeted, chip-based Pyrosequencing"http://www.biomedcentral.com/1471-2180/7/108BMC Microbiology 2007;7():108-108.Published online 30 Nov 2007PMCID:PMC2244631. by amplification of 16S rDNA using universal primers. These fragments are then sequenced with high-throughput Pyrosequencing. Each read is queried against a database of known 16S rDNA sequence (mostly obtained from the ) using the program and assigned to the most specific and confident node in the phylogeny. Accumulating all the reads in this fashion yields a weighted phylogenetic tree characterizing the bacterial content of the sample.
DOI:10.6084/m9.figshare.21956