Additional file 3 of Macroglossia and less advanced dystrophic change in the tongue muscle of the Duchenne muscular dystrophy rat

Additional file 3: Supplementary Fig. 3. Western blot of molecules involved downstream of Akt-mTOR pathway in the tongue muscle at 6-month-old. Two bands (doublet) were detected by the anti-4E-BP1 antibody. The upper (p-4E-BP1 (high)) and lower (p-4E-BP1 (low)) bands correspond to highly phosphorylated 4E-BP1 and non- or lower-phosphorylated 4E-BP1, respectively. α-tubulin was used as an internal control. p-Akt (S473), Akt phosphorylated at Ser473. p-S6K (T389), S6 kinase phosphorylated at Thr389. p-S6 (S240/244), S6 ribosomal protein phosphorylated at Ser240 and Ser244. n, the number of samples.