Additional file 4 of Corncob structures in dental plaque reveal microhabitat taxon specificity

Additional file 4. Probe set validation matrices showing hybridization of pure cultures with complete probe sets. For each probe set, image acquisition and linear unmixing of all cultures were carried out under consistent conditions using Zeiss ZEN software. Images were imported into FIJI and the range of display intensities was kept constant for each fluorophore (each column in the figure). In addition to the species probes shown in Fig. 1 and Additional File 1, probes and their targets and fluorophores are as follows: Str405 [62] targeting genus Streptococcus, labeled with Rhodamine Red X (RRX); Por1160 [64] targeting the gingivalis group of genus Porphyromonas, labeled with Alexa 555; Pas111 [64] targeting family Pasteurellaceae, labeled with Dy615; Cor633 [12] targeting genus Corynebacterium, labeled with Dy490 (part A) or Atto 620 (part B); probe Cmat175 [12] targeting species C. matruchotii, labeled with Atto 655. All probes hybridized with their expected targets and showed negligible cross-hybridization to unexpected targets. (At532: Atto 532; TRX: Texas Red X).