Additional file 1 of Corncob structures in dental plaque reveal microhabitat taxon specificity

Additional file 1. Validation of new oligonucleotide probes targeting subsets of the genus Streptococcus. For each probe, a full list of oral Streptococcus species and their matches and mismatches to the probe is shown (top), along with images showing the intensity of signal imaged after hybridization of pure cultures of cells to the probes. Each newly designed probe was hybridized simultaneously with existing probes targeting genus Streptococcus and most Bacteria as controls. 15 pure cultures were hybridized; 8 are shown here and the remaining 7 are shown in Fig. 1. A) Probe Scri995 targeting S. cristatus; B) probe Sgor63 targeting S. gordonii; C) probe Smit371 targeting S. mitis and its close relatives and S. cristatus; D) probe Smit651 targeting S. mitis and its close relatives. For each probe set, image acquisition and linear unmixing of all cultures were carried out under the same conditions using Zeiss ZEN software. Images were imported into FIJI and the range of display intensities was kept constant for each fluorophore (each column in the figure); for cultures where the fluorescence was dim in all channels, the display range was then additionally adjusted by a constant factor for all images in the row to improve visibility of cells; these rows are marked (*). All probes hybridized with their expected targets and showed negligible cross-hybridization to unexpected targets. (RRX: Rhodamine Red X; TRX: Texas Red X).