Effect of aging on murine CD8+ T cell chemotaxis response to MIP-1α

Copyright information:Taken from "The effect of aging and caloric restriction on murine CD8+ T cell chemokine receptor gene expression"http://www.immunityageing.com/content/4/1/8Immunity & ageing : I & A 2007;4():8-8.Published online 14 Nov 2007PMCID:PMC2200663. The indicated concentrations of the chemokine were placed in the lower chamber of a Costar Transwell system. Equal number of freshly isolated young (3–4 months) or old (18–20 months) CD8+ T cells was then placed in the upper chamber of the insert, and the number of cells in the upper and lower chambers counted 5 hours later using a Coulter counter. The results are expressed as percent transmigration and represent the mean ± SD of triplicate determinations. P ≤ 0.05 is considered significant. The results are representative of 3 independent experiments.