Additional file 2 of Genetic control of neuronal activity enhances axonal growth only on permissive substrates

Additional file 2: Figure S2. ChR2 expression and growth after CST axotomy in Thy1-ChR2 mice. A. While some layer II–III neurons express ChR2, expression is concentrated in layer V neurons. Scale bar: 100 μm. B. Schematic representation of the quantification in C. The spinal cord was divided in grey matter and white matter for analysis. C. Tracer intensity quantification of injured stimulated and non-stimulated Thy1-ChR2 mice at 0.5 mm pre-injury and post-injury. Optogenetic stimulation of the CST resulted in increased sprouting in the grey matter pre-injury, but not post-injury. a.u.: arbitrary units. Data are expressed as mean tracer intensity ± s.e.m (n = 5 spinal cords). **p < 0.01 denotes significant differences in Student’s t-Test.