Impact of deletion on cell wall proteins

Copyright information:Taken from "A mutant with strongly reduced lipoteichoic acid (LTA) content: LTA governs bacterial surface properties and autolysin activity"Molecular Microbiology 2007;65(4):1078-1091.Published online Jan 2007PMCID:PMC2169524.© 2007 The Authors; Journal compilation © 2007 Blackwell Publishing Ltd A. Surface protein patterns of cells grown to logarithmic (left) or stationary (right) phase. SA113 wild-type and SA113 Δ mutant strains did not differ in overall amounts of cell wall proteins. Ten micrograms of surface proteins of the indicated SA113 strains were separated in 10% polyacrylamide gels and stained with Coomassie brilliant blue R-250. B. Zymographic analyses. Surface proteins (20 μg) of SA113 log-phase bacteria were separated in 10% SDS-polyacrylamide gels containing heat-killed (), SA113 wild type (wt) or SA113 Δ mutant cells (Δ). Active bacteriolytic enzymes appear as clear zones in the opaque gels. The inverse pictures are shown. C. Spontaneous bacterial autolysis. Data represent the means ± SD of three independent experiments.