Additional file 2 of DNA methylation alterations across time and space in paediatric brain tumours

Additional file 2. A) Boxplot visualising the classification score for each methylation superfamily. The classification score ranges between 0 and 1, where ≥0.9 is considered a successful classification (indicated by dashed line). B) The superfamily classification score (y-axis) for all samples (spatial and temporal) versus the tumour cell content (x-axis). The samples are coloured according to their methylation superfamily. C) Hierarchical clustering of the top 10000 most variable CpG sites and D) multi-dimensional scaling (MDS) plot of the top 20000 most variable CpG sites of all samples mainly cluster samples according to their methylation superfamily. The legend in D applies to all subfigures. Abbreviations: Choroid plexus – Choroid plexus tumours. Diffuse glioma – Diffuse glioma, MAPK altered, cell cycle-activated. LGG – low-grade glial/glioneuronal/neuroepithelial tumours. Neuroep. PATZ – Neuroepithelial tumour with PATZ1 fusion. Embryonal tumour – Other embryonal tumours. Diffuse HGG – Paediatric-type diffuse high-grade gliomas.