Genetic determinants of hyaloid and retinal vasculature in zebrafish-4

Copyright information:Taken from "Genetic determinants of hyaloid and retinal vasculature in zebrafish"http://www.biomedcentral.com/1471-213X/7/114BMC Developmental Biology 2007;7():114-114.Published online 15 Oct 2007PMCID:PMC2169232.o facilitate quantification of the vessel branches. A black circumferential line demarks half the distance between the optic disc and the annular peripheral vein at different retinal regions (MID- retina). The number of main branches radiating from the optic disc and the number of vessels crossing the mid-retina are higher in mutants (n = 18) than in wild type retinas (n = 24). The average number of branch points per vessel is significantly decreased in (n = 5) versus wildtype (n = 5) retinas. The number of branch points per 9 randomly chosen vessels in each retina was counted from outside in. To avoid bias, the nearest vessel was chosen every 40° of retinal circumference, starting from the most ventral poin. A significant reduction in the distance from the optic disc branches to the secondary branches, but not to the tertiary branches, is observed in vessels (n = 40) versus wildtypes (n = 22).