Genetic determinants of hyaloid and retinal vasculature in zebrafish-2

Copyright information:Taken from "Genetic determinants of hyaloid and retinal vasculature in zebrafish"http://www.biomedcentral.com/1471-213X/7/114BMC Developmental Biology 2007;7():114-114.Published online 15 Oct 2007PMCID:PMC2169232. overlying the inner limiting membrane (ILM) of the retina in adult zebrafish. . Ultrastructure of capillary. The basal lamina (BL) is in contact with the ILM enclosing pericytes (PE) and the vascular endothelium. . Typical structural features of pericytes, (GA), rough endoplasmic (RER) and large membrane bound vesicles. . Vitreal space lined by pericyte overlying endothelial cells (EC) which display interdigitating junctional complexes (arrows). . Multiple vesicles (arrows) from 20 to 250 nm contacting the inner limiting membrane of the retina indicate active interaction between the vessels and the ganglion cell layer (at the Müller endfeet). . Vascular (top) and ganglion cell layer interface (bottom). A vesicle (left panel) apparently separated from the cell membrane, fuses with the cell membrane when the section is tilted by 43° (right panel) suggesting transcellular transport. . Interendothelial junctions are significantly more open in the senescent fish. Distance between endothelial cells at tight (asterisks) and adherent (arrows) junctions were measured in different peripheral and central areas of retinas from senescent and young adult fish. . Histology of a 5 dpf zebrafish eye. . EM image of a 5 dpf larval eye illustrating the relationship of hyaloid vessels (encircled by a red line) to both the lens and the retina. . Higher magnification of the hyaloid vessel confirms its tight attachment to the lens and looser contact to the retina (arrows). .