Optimised linear templates for expression

Copyright information:Taken from "A systematic approach for testing expression of human full-length proteins in cell-free expression systems"http://www.biomedcentral.com/1472-6750/7/64BMC Biotechnology 2007;7():64-64.Published online 3 Oct 2007PMCID:PMC2131746. Three kinds of linear PCR-products were generated to investigate the effect on protein expression rate and yield. (i) C-terminal wild type with C-terminal 6xHis-tag (ii) C-terminal mutant with C-terminal 6xHis-tag and inserted silent mutation at the N-terminus (iii) N-terminal wild type with N-terminal 6xHis-tag and no attachment sites (att-sites); For the C- and N-terminal wild type template the ORF is identical to the original ORF in the plasmid DNA.