Additional file 1 of Cleavage of tropomodulin-3 by asparagine endopeptidase promotes cancer malignancy by actin remodeling and SND1/RhoA signaling

Additional file 1: Fig. S1. Relationship between AEP, Tmod3 and lysosome. (A) Representative images showing lysosomal status of A172 and U87-MG cells treated with shCtrl or shAEP (left panel) with quantification (right panel). Scale bar, 100 μm. (B) Representative confocal images showing the colocalization of AEP(red), Tmod3 (green) and LAMP2 (violet) in A172 cells by immunofluorescent staining (left panel, yellow arrows indicated the colocalization of AEP and Tmod3; white arrows indicated the colocalization of AEP, Tmod3 and LAMP2). Colocalization tracer profile along the line (white arrow)is indicated as merged image (right panel). Scale bar, 10 μm. NC=negative control, cells expressing scramble shRNA. KD=knockdown, cells expressing AEP shRNA.*P