Additional file 3 of Dysregulated myosin in Hermansky-Pudlak syndrome lung fibroblasts is associated with increased cell motility

Additional file 3: Fig S3. A Western blot analysis of Myosin IIB in HPSLF (n = 3 technical replicates) treated with vehicle (DMSO) or with SB202190 (5 µM). β-Actin was used as a loading control. B Ratio of Myosin IIB to β-Actin density expressed as fold-change relative to DMSO. C Western blot analysis of phospho-p38 and p38 in NLF (n = 3 technical replicates) transfected with control or 2 different HPS1 siRNA constructs. β-Actin was used as a loading control. D Ratio of phospho p38 to p38 Density; the ratio of each HPS1 transfected NLF was compared to its own control siRNA transfected cell. E Western blot analysis of phospho-p38 and p38 in NLF (n = 3 technical replicates) transfect with control or HPS4 siRNA. β-Actin was used as a loading control. (F) Ratio of phospho p38 to p38 Density. Data are expressed as mean ± SEM of three independent experiments. Data analyzed using a student’s t-test *P < 0.05, **P < 0.01, ***P < 0.001, ***P < 0.0001