Additional file 3 of Combined inhibition of EZH2 and ATM is synthetic lethal in BRCA1-deficient breast cancer

Additional file 3: Fig. S3. Concentration optimization for combined GSK126/AZD1390 treatment. Determination of optimized synergistic compound concentrations for the prioritized drug combination GSK126/AZD1390 resulting in maximal synergistic effects. Cells were permanently exposed to single agent or combined treatment as indicated. After 72 hours treatment, cell viability was measured using CellTiter-Glo assay. (A) Dose-response curves of increasing concentrations of GSK126 (1.25, 2.5, 5, 7.5, 10 µM) alone (orange) or in combination with a fixed concentration of AZD1390 (1, 2, 3, 4, 5 µM) (purple). (B) Dose-response curves of AZD1390 (1, 2, 3, 4, 5 µM) alone (orange) or in combination with a fixed concentration of GSK126 (1.25, 2.5, 5, 7.5, 10 µM) (purple). Black arrows indicate inhibitor concentrations with maximal synergistic effect after 72 hours treatment. Statistical significance was tested by one-way ANOVA with Tukey multiple comparison test.