Additional file 8 of Novel App knock-in mouse model shows key features of amyloid pathology and reveals profound metabolic dysregulation of microglia

Additional file 8: Supplementary Figure 8. Comparative analysis of microglia transcriptome. a Absolute expression (log2 counts per million) of cell type specific markers shows strong enrichment and purity of microglia cell population analyzed in this Study. b Log2 fold change of methoxy-X04 (-) AppSAA +/+ microglia vs methoxy-X04 (-) WT microglia (x-axis) vs log2 fold change of methoxy-X04 (+) AppSAA +/+ microglia vs methoxy-X04 (-) WT (y-axis). Only genes with log2 fold change >= 1.2 and FDR = 1. d comparison of log2 fold changes of genes identified in AppSAA KI/KI methoxy-X04 (+) microglia vs WT (x-axis) and 5xFAD methoxy-X04 (+) microglia vs WT (y-axis) in Grubman et al. Spearman’s rho: 0.66, p-value < 1e-10. e Functional enrichment scores of the union of top 10 Gene Ontology Biological Process categories identified in methoxy (+) microglia vs WT in AppSAA KI/KI mice (orange) and 5xFAD mice from Grubman et al. (purple). Bars indicate -log10(q-value) from an overrepresentation analysis of upregulated genes in each of the ontology terms.