Additional file 5 of Novel App knock-in mouse model shows key features of amyloid pathology and reveals profound metabolic dysregulation of microglia

Additional file 5: Supplementary Figure 5. Evidence of CAA pathology in AppSAA mice at 8 and 16 months of age. a Representative images of Aβ deposition in AppSAA +/+, AppSAA KI/+, and AppSAA KI/KI mice at 16 months of age; AppSAA KI/+ and AppSAA KI/KI exhibit parenchymal Aβ plaque deposition (examples indicated with yellow arrowheads for AppSAA KI/+) and Aβ deposition associated with leptomeningeal vessels (CAA; examples indicated with white arrows for both AppSAA KI/+ and AppSAA KI/KI). Scale bars = 1 mm. b Representative confocal images showing accumulation of Aβ surrounding leptomeningeal (pial) vessels (endothelial cells labeled by CD31 and smooth muscle cells labeled by alpha-smooth muscle actin, consistent with putative arteries/arterioles) in AppSAA mice at 8 and 16 months of age. Scale bars = 50 μm (left images and magnified views). c-e Super resolution confocal images from AppSAA KI/KI mice 16 months of age showing Aβ accumulation in a penetrating parenchymal vessel (c), branching leptomeningeal vessel in the ambient cistern (d) and a leptomeningeal vessel (e), scale bars = 20 μm for images across (c-e).