Additional file 2 of Lack of STAT6 enhances murine acute lung injury through NLRP3/p38 MAPK signaling pathway in macrophages

Additional file 2: Fig. S2 Uncropped full-length blots were included for Fig. 1D and Fig. 3C.20 µg protein samples were resolved on 15 wells and 1.5 mm thickness of 10% SDS-PAGE gel. After running for 1 h at 100 V, the resolved protein was transferred onto polyvinylidene fluoride membranes. The blots were then cut around the expected protein size, according to protein size marker and incubated with indicated primary antibodies. The blots were stripped for multiple hybridization. Images show full-length of original blots with visible membrane edges. In Fig. 1D, BMDMs from WT and STAT6 KO mice were treated with or without 500 ng/ml LPS for 24 hrs. The expression of total STAT6 in the treated cells were analyzed. GAPDH was internal loading control. In Fig. 3C, the expression of NLRP3, ASC, p-p38 MAPK, total p38 MAPK, p62 and LC3 in the lung tissues of mice with ALI was analyzed. GAPDH and β-Tubulin were internal loading controls. The lanes used in Fig. 1D and Fig. 3C were labeled