Additional file 1 of Myt1l haploinsufficiency leads to obesity and multifaceted behavioral alterations in mice

Additional file 1. Figure S1: Molecular characterization of Myt1l expression in mutant mice. (A) Western blot analysis of Myt1l expression in WT or Myt1l HET mouse embryo brains. RabGDI was used as a loading control. (B) Quantification of Myt1l protein expression. The Myt1l bands signal intensity was normalized to that of RabGDI and then to the value of 1.0 for WT (N=3/genotype, one sample t test; P=0.022; error bar, s.d.). (C) qRT-PCR measuring Myt1l transcript levels in brains of WT and HET males (16 month old), normalized to MAP2 (N=3/genotype, t test; ns, not significant, P>0.05; error bars, s.d.). (D) Uncropped Western blot of the blot shown in Suppl. Figure S1A. (E) Uncropped Western blot of the blot shown in Figure 1C.