Cell displaying dynamic centroid motion with more static uropod. from T cell morphodynamics reveal periodic shape oscillations in three-dimensional migration

Cell displaying dynamic centroid motion with more static uropod. from T cell morphodynamics reveal periodic shape oscillations in three-dimensional migration

Primary murine T cell isolated from mT/mG x OT1 (membrane reporter) mouse embedded in 3D collagen matrix (not visualised) imaged by Lattice Light-Sheet microscopy. Top: Raw fluorescence signal. Bottom: Post-segmentation surface rendering. Surface rendering was only retained for downstream analysis f...

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Hauptverfasser: Cavanagh, Henry, Kempe, Daryan, Mazalo, Jessica K., Biro, Maté, Endres, Robert G.
Format: Video
Sprache:eng
Schlagworte:
Biophysics
Computational Biology
FOS: Biological sciences
Systems Biology
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Zusammenfassung:Primary murine T cell isolated from mT/mG x OT1 (membrane reporter) mouse embedded in 3D collagen matrix (not visualised) imaged by Lattice Light-Sheet microscopy. Top: Raw fluorescence signal. Bottom: Post-segmentation surface rendering. Surface rendering was only retained for downstream analysis from timeframes where the entire cell is contained within the field-of-view. Time shown as h:min:s. Scale bar: 5 µm.
DOI:10.6084/m9.figshare.19615102