Additional file 3 of Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression
Additional file 3: Figure S3. Selective sorting of hY4F into EVs inhibits the proliferation, migration, and invasion of lung cancer cells. (A) H1975 cells were incubated with hY4F mimic/NC-overexpressing A549 EVs for 48 h and then the level of hY4F was examined by qPCR. (B) The EdU assay was perform...
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| creator | Li, Chuang Wang, Wei Sun, Yuting Ni, Yifan Qin, Fang Li, Xiaolu Wang, Tao Guo, Mingxiong Sun, Guihong |
| description | Additional file 3: Figure S3. Selective sorting of hY4F into EVs inhibits the proliferation, migration, and invasion of lung cancer cells. (A) H1975 cells were incubated with hY4F mimic/NC-overexpressing A549 EVs for 48 h and then the level of hY4F was examined by qPCR. (B) The EdU assay was performed to assess the effect of hY4F-overexpressing A549 EVs on H1975 cell proliferation (at 48 h after treatment). (C) Flow cytometry using Annexin V-FITC/PI staining was performed on H1975 cells incubated with hY4F-enriched A549 EVs to analyze apoptosis (at 48 h after treatment). (D-E) The effect of hY4F-enriched A549 EVs on H1975 migration and invasion were assessed by transwell assay (at 48 h after treatment). Data from three independent experiments are shown as the mean ± SD (error bars). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). NC: negative control. |
| doi_str_mv | 10.6084/m9.figshare.19581870 |
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Selective sorting of hY4F into EVs inhibits the proliferation, migration, and invasion of lung cancer cells. (A) H1975 cells were incubated with hY4F mimic/NC-overexpressing A549 EVs for 48 h and then the level of hY4F was examined by qPCR. (B) The EdU assay was performed to assess the effect of hY4F-overexpressing A549 EVs on H1975 cell proliferation (at 48 h after treatment). (C) Flow cytometry using Annexin V-FITC/PI staining was performed on H1975 cells incubated with hY4F-enriched A549 EVs to analyze apoptosis (at 48 h after treatment). (D-E) The effect of hY4F-enriched A549 EVs on H1975 migration and invasion were assessed by transwell assay (at 48 h after treatment). Data from three independent experiments are shown as the mean ± SD (error bars). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). 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Selective sorting of hY4F into EVs inhibits the proliferation, migration, and invasion of lung cancer cells. (A) H1975 cells were incubated with hY4F mimic/NC-overexpressing A549 EVs for 48 h and then the level of hY4F was examined by qPCR. (B) The EdU assay was performed to assess the effect of hY4F-overexpressing A549 EVs on H1975 cell proliferation (at 48 h after treatment). (C) Flow cytometry using Annexin V-FITC/PI staining was performed on H1975 cells incubated with hY4F-enriched A549 EVs to analyze apoptosis (at 48 h after treatment). (D-E) The effect of hY4F-enriched A549 EVs on H1975 migration and invasion were assessed by transwell assay (at 48 h after treatment). Data from three independent experiments are shown as the mean ± SD (error bars). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). NC: negative control.</description><subject>Biochemistry</subject><subject>Biophysics</subject><subject>Cancer</subject><subject>Cell Biology</subject><subject>Chemical Sciences not elsewhere classified</subject><subject>FOS: Biological sciences</subject><subject>FOS: Health sciences</subject><subject>Genetics</subject><subject>Infectious Diseases</subject><subject>Medicine</subject><subject>Molecular Biology</subject><subject>Pharmacology</subject><subject>Physical Sciences not elsewhere classified</subject><subject>Virology</subject><fulltext>true</fulltext><rsrctype>image</rsrctype><creationdate>2022</creationdate><recordtype>image</recordtype><sourceid>PQ8</sourceid><recordid>eNqdjz1Ow0AQhd1QoMANKOYCMbZiwC4DAlFRAAWpVsPu2B5pf6LZSYTvw0GxEbkA1Zs3ek9PX1Fc1VV5W7XNdejKnoc8olBZdzdt3d5V58X31jlWThE99OwJNpB6eCNPVvlIkJMoxwEwOshkhZbsEhl3Dby-bKEXHAJFzcBRE9CXClry_uBR4EiZracMgRyjkoPPab51nPyv291_1DC39pJCUgJ_mKcsRkuy_AahnOe9i-KsR5_p8k9XRfP0-P7wvHaoaFnJ7IUDymTqyiywJnTmBGtOsJt_1n4AT2prgg</recordid><startdate>20220412</startdate><enddate>20220412</enddate><creator>Li, Chuang</creator><creator>Wang, Wei</creator><creator>Sun, Yuting</creator><creator>Ni, Yifan</creator><creator>Qin, Fang</creator><creator>Li, Xiaolu</creator><creator>Wang, Tao</creator><creator>Guo, Mingxiong</creator><creator>Sun, Guihong</creator><general>figshare</general><scope>DYCCY</scope><scope>PQ8</scope><orcidid>https://orcid.org/0000-0001-7680-0272</orcidid></search><sort><creationdate>20220412</creationdate><title>Additional file 3 of Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression</title><author>Li, Chuang ; Wang, Wei ; Sun, Yuting ; Ni, Yifan ; Qin, Fang ; Li, Xiaolu ; Wang, Tao ; Guo, Mingxiong ; Sun, Guihong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_6084_m9_figshare_195818703</frbrgroupid><rsrctype>images</rsrctype><prefilter>images</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biochemistry</topic><topic>Biophysics</topic><topic>Cancer</topic><topic>Cell Biology</topic><topic>Chemical Sciences not elsewhere classified</topic><topic>FOS: Biological sciences</topic><topic>FOS: Health sciences</topic><topic>Genetics</topic><topic>Infectious Diseases</topic><topic>Medicine</topic><topic>Molecular Biology</topic><topic>Pharmacology</topic><topic>Physical Sciences not elsewhere classified</topic><topic>Virology</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Chuang</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Sun, Yuting</creatorcontrib><creatorcontrib>Ni, Yifan</creatorcontrib><creatorcontrib>Qin, Fang</creatorcontrib><creatorcontrib>Li, Xiaolu</creatorcontrib><creatorcontrib>Wang, Tao</creatorcontrib><creatorcontrib>Guo, Mingxiong</creatorcontrib><creatorcontrib>Sun, Guihong</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Li, Chuang</au><au>Wang, Wei</au><au>Sun, Yuting</au><au>Ni, Yifan</au><au>Qin, Fang</au><au>Li, Xiaolu</au><au>Wang, Tao</au><au>Guo, Mingxiong</au><au>Sun, Guihong</au><format>book</format><genre>unknown</genre><ristype>GEN</ristype><title>Additional file 3 of Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression</title><date>2022-04-12</date><risdate>2022</risdate><abstract>Additional file 3: Figure S3. Selective sorting of hY4F into EVs inhibits the proliferation, migration, and invasion of lung cancer cells. (A) H1975 cells were incubated with hY4F mimic/NC-overexpressing A549 EVs for 48 h and then the level of hY4F was examined by qPCR. (B) The EdU assay was performed to assess the effect of hY4F-overexpressing A549 EVs on H1975 cell proliferation (at 48 h after treatment). (C) Flow cytometry using Annexin V-FITC/PI staining was performed on H1975 cells incubated with hY4F-enriched A549 EVs to analyze apoptosis (at 48 h after treatment). (D-E) The effect of hY4F-enriched A549 EVs on H1975 migration and invasion were assessed by transwell assay (at 48 h after treatment). Data from three independent experiments are shown as the mean ± SD (error bars). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). 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| subjects | Biochemistry Biophysics Cancer Cell Biology Chemical Sciences not elsewhere classified FOS: Biological sciences FOS: Health sciences Genetics Infectious Diseases Medicine Molecular Biology Pharmacology Physical Sciences not elsewhere classified Virology |
| title | Additional file 3 of Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression |
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